Abstract: In order to study the effects of soaking seeds with different concentrations of gibberellin on seed germination of Radix bupleuri, to measure and analyze the variation of 4 endogenous hormone contents during seed germination process, and to provide theoretical basis and feasible method for seed germination regulation and seed germination rate improvement of R. bupleuri, the seeds were treated with 0 mg·L^-1, 50 mg·L^-1, 100 mg·L^-1, 150 mg·L^-1 and 200 mg·L^-1 gibberellin solution, and the contents of ABA, GA₃, IAA and ZR in seeds during germination were determined by HPLC. The results showed that the germination potential and germination rate of R. bupleuri seeds soaked with different concentrations of gibberellin were significantly different, and the germination potential（30.1%） and germination rate（64.5%） of seeds soaked with 150 mg·L^-1 were the highest. Compared with the control, the contents of ABA and IAA in other soaking treatments were significantly decreased, while the contents of GA₃ and ZR were significantly increased. During the germination process, IAA+GA₃+ZR/ABA ratio of all soaking seeds showed a “rise-decline-rise” change, and the ratio of other soaking seeds was significantly higher than that of control group, and the highest value was 150 mg·L^-1 soaking seeds. The results showed that the contents of ABA, GA₃, IAA and ZR in seeds of R. bupleuri showed different changes during the germination process, and gibberellin soaking seeds may promote seed germination by regulating the increase of IAA+GA₃+ZR/ABA ratio.