Abstract: In order to select the best method for Jerusalem artichoke（Helianthus tuberosus）seeds initiation, sexual reproduction seeds of Jerusalem artichoke were studied by low temperature, alternating wet and dry, warmsoup soaked. The results showed that the best method of initiating Jerusalem artichoke seeds waswarm soup soaking, the germination rate, germination power and germination index reached 53.33%,15.56% and 1.93 respectively. The seeds studied by sodium hydroxide soaked and gibberellicacid soaked, results showed that the best method of initiating Jerusalem artichoke seeds was 30 g·L^-1 sodium hydroxide soaking, germination rate, germination power and germination indexreached 38.89%,10.0% and 1.60 respectively. The seeds studied by sodium chloride induced and gibberellic acid induced backdrying. Meanwhile the best method of initiating Jerusalem artichoke seeds was 20 g·L^-1 sodium chloride induced back drying and the germination rate, germination power and germination index reached 44.44%,14.4% and 1.40 respectively. By comprehensive comparison, results showed that the best method of initiating Jerusalem artichoke seeds was warm soup soaking. This study provides reference for the utilization and development of Jerusalem artichoke seeds in the future.