Abstract: The application potential of Yunrui 05-292 as a variety and parent was systematically evaluated by family evaluation and homology analysis of the 12 th round of regional test in Yunnan Province and the regional breeding hybrid combinations in Yunnan Province. 1) Compared with the varieties（lines） tested in the 12 th round of regional test in Yunnan Province, yunrui parent lines Yunrui 05-292 and Yunzhe 06-160 were comparable to the introduced varieties Sp 80-3280 and Sp 81-3250 with the best comprehensive performance in this test, and superior to other introduced and self-bred varieties（lines） tested in Yunnan; 2) Yunrui 05-292 has a large number of effective stems, strong rooting capacity, uniform and orderly cane stems, and has good application potential for mechanical harvesting varieties; 3) Evaluation results of type 17 combination family: Yunrui 05-292, Yunrui 05-292× Yunrui 05-51, Yunrui 99-601, CP 94-1100× Yunrui 99-601, Yunrui 10-299, ROC 6× Yunrui 10-299 Yunrui 10-463, Yunzhe 05-51× Yunrui 10-463 could be used as the male parent and combination of high sugar breeding. 4) The evaluation results of type 15 combination in Yunnan Region breeding showed that yunzhe 05-51× Yunrui 05-292 had good progenies, yunrui 05-292 and Yunzhe 05-51 could be used as excellent parents in sugarcane breeding.