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射干种子萌发的影响因素及其对NaCl胁迫的响应

Influence Factors of Seed Germination of Belamcanda chinensis and Its Response to NaCl Stress

  • 摘要: 通过统计种子潜在产量、生活力、抑制物萌发以及多种处理条件下的发芽指标等,明确射干种子的适宜萌发条件。探究种子耐NaCl胁迫的幅度范围。结果表明,射干种皮透水性良好。自然环境贮藏条件下种子生活力在2年内下降幅度不显著;射干种皮浸提液对小白菜种子发芽有显著抑制作用;昼夜变温处理和层积处理后射干种子的发芽率和发芽势显著提高;适宜射干种子发芽的便捷处理方法为:低温层积处理30 d后,25℃(8 h)/15℃(16 h)昼夜变温条件下萌发;浓度为0~200 mmol/L的NaCl溶液对射干种子的各项萌发指标影响不显著。NaCl浓度在300 mmol/L以上时,种子的各项发芽指标随胁迫程度的提高均呈显著降低趋势。预测射干种子NaCl胁迫浓度的临界值和极限值分别为337 mmol/L和457 mmol/L。

     

    Abstract: In this paper, the suitable germination conditions of Belamcanda chinensis seeds were investigated by the statistics of seed potential yield, viability, inhibitory germination and germination under various treatment conditions, and the range of seed tolerance to NaCl stress was also explored. The results showed that the water permeability of B. chinensis seed coat was good, and seed viability did not decrease significantly in 2 years under natural storage conditions. The extracts from the seed coat had significant inhibition on the germination of pakchoi seeds, and the germination rate and germination potential of B. chinensis seeds were significantly increased after diurnal temperature and stratification treatments. The convenient treatment method suitable for germination of B. chinensis seeds was as follows after 30 days of low-temperature stratification, germination was conducted at 25 ℃(8 h)/15 ℃(16 h) day and night. In addition, the concentration of 0-200 mmol/L NaCl solution had no significant effect on the germination indexes of the seeds, while the germination index of seeds decreased significantly with the increase of stress degree when NaCl concentration was above 300 mmol/L. The threshold and limit values of NaCl stress concentration were 337 mmol/L and 457 mmol/L, respectively.

     

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