Abstract: Ear type is an important character of wheat ear, which is closely related to wheat yield and quality. In this study, ethyl methylate（EMS） was used to treat the new wheat line Hekeda 527（KD 527）, and a series of ear type mutants were selected from the EMS-treated offsprings of KD 527 through the identification of agronomic traits for many years, and genetic analysis of ear type and its effect evaluation were carried out. The results showed that 1) A mutant library containing 15 different ear types was constructed. Compared with the wild type KD 527, all the mutants showed lower plant height, panicle length, grain number per panicle and 1 000-grain weight except ear type. At the same time, the organs of flag leaf, lower panicle and awn were abnormal. 2) The genetic analysis of ear type in the self-breeding progenies of different ear type mutants showed that all the self-breeding progenies of panicle type mutants had the separation of normal ear type and twisted ear type. Based on the statistical analysis of 238 self-crossing individual plants of different mutants, 69 plants had normal ear type, 169 plants had twisted ear type（37 plants had extreme twisted ear type）. Further, the X~2 test showed that the ratio of twisted ear type to normal ear type followed the rule of 3∶1 separation, which implied normal panicle type was inherited by single recessive gene. 3) The statistical results of agronomic traits per plant of self-crossing progeny of mutant showed that the average plant height, spike length and 1 000-grain weight of normal ear type were 53.1 cm, 8.42 cm and 55.9 g, while the average plant height, spike length and 1 000-grain weight of twisted ear type were 43.1 cm, 6.20 cm and 35.2 g, respectively. The differences of plant height, panicle length and 1 000-grain weight between normal and twisted ear were extremely significant, while the single plant of extreme twisted ear showed no heading and no fruit. The constructed mutants of different ear types provided germplasm basis for genetic analysis of ear types and analysis of genetic effects in wheat, and had important application value for genetic improvement of spike related traits in wheat.