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河北省旱碱麦品质和抗性相关基因的KASP标记检测

Detection of Quality and Resistance-related Genes by KASP Markers in Dry-alkali Wheat of Hebei Province

  • 摘要: 利用12个小麦品质、抗病和抗逆等相关的KASP标记,对河北省育成的部分旱碱麦和农家种(54份)进行KASP标记检测。结果表明,在品质方面,有15份材料含有Glu-D1基因的高分子量麦谷蛋白亚基5+10,有32份材料是Glu-A1基因的Ax1 or Ax2*强筋单倍型。在抗病性方面,有25份含有抗叶锈病基因Lr34,有27份含有抗赤霉病基因Fhb1。在抗穗发芽方面,有24份材料含TaSdr-B1基因的TaSdr-B1a抗穗发芽单倍型;有47份材料含有TaPHS1基因的Rio Blanco type抗穗发芽单倍型;有9份材料含有TaMoc-A1基因的Hap-H抗穗发芽单倍型。在抗旱性方面,有44份材料含有COMT-3B基因的3Ba抗旱单倍型;有12份材料含有Dreb-B1基因的TaDREB-B1a抗旱单倍型;有49份材料含有TaSST-4A基因的A2a抗旱单倍型。

     

    Abstract: In this paper, 12 KASP markers related to wheat quality, disease resistance and stress tolerance were used to detect the KASP markers in 54 dry-alkali wheat and farm wheat grown in Hebei province. The results showed that, in terms of quality, 15 materials contained glutenin subunit(5+10) with high molecular weight encoded by Glu-D1, and 32 materials were strong gluten haplotype containing Ax1 or Ax2* of Glu-A1 gene. In terms of disease resistance, 25 of materials contained leaf rust resistance gene Lr34, and 27 contained fusarium head blight-resistant gene Fhb1. In terms of resistance to sprouting, 24 materials contained Tasdr-b1a-containing anti-sprouting haplotype of TaSdr--B1 gene and 47 materials contained Rio Blancotype-containing anti-sprouting haplotype of TaPHS1 gene, and the other 9 materials contained Hap-H-containing anti-sprouting haplotype of TaMoc-A1 gene. Additionally, in terms of drought resistance, there were 44 materials contained 3 Ba-containing drought-resistant haplotype of COMT-3B gene and 12 materials contained TaDREB-B1a-containing drought-resistant haplotype of Dreb-B1 gene, while there 49 materials contained A2a-containing drought-resistant haplotype of TaSST-4A gene.

     

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