Abstract: To construct the fingerprint of maize hybrid Beiqing 340 and its parents, 40 pairs of maize core primers were used to analyze Beiqing 340 by SSR molecular marker technology, and seven pairs of primers with clear bands and good repeatability were screened out, including umc 1147 y 4, bnlg 1671 y 17, umc 1999 y 3, phi 299852 y 2, umc 1936 k 4, umc 2163 w 3, bnlg 2291 k 4. And, the fingerprint was constructed into digital fingerprint with “0” and “1”. At the same time, the probability of having the same fingerprint was very low, which was 2.98×10^-8. Therefore, these 7 pairs of primers could be used to identify the authenticity of Beiqing 340 and its parents.