Abstract: In order to clarify the genetic basis of the new wheat variety Xinmai 45 of high-quality and strong gluten, the whole genome of Xinmai 45 and its parents Xinmai 26 and Jimai 20 were scanned and analyzed by wheat 55 K SNP chip to explore the genetic composition of Xinmai 45.The results showed that the genetic contribution rates of the parents Xinmai 26 and Jimai 20 to Xinmai 45 were quite different at the genome-wide level.The genetic contribution rate of Xinmai 26 to Xinmai 45 was 62.28%,which was higher than that of Jimai 20,with the contribution rate of 37.72%;The genetic contribution rate of Xinmai 26 in B genome and D genome was higher than that of Jimai 20,while Xinmai 45 inherited more genetic material of Jimai 20 in A genome; On different chromosomes, the genetic contribution rate of both parents to Xinmai 45 was quite different, of which the genetic contribution rate of Xinmai 26 on 1 B,1 D,2 B,2 D,4 B,5 A,5 B,5 D and 7 B chromosomes was much higher than that of Jimai 20,while the genetic contribution rate（90.71%） of Jimai 20 on chromosome 2 A was much higher than that of Xinmai 26;Among the larger genetic segments of the chromosomes of both parents of Xinmai 45,Xinmai 26 had more larger genetic segments than Jimai 20,while Xinmai 45 obtained more large genetic fragments of Jimai 20 chromosomes on chromosomes 2 A,6 A and 6 B,and Xinmai 45 had obvious biased selection.This study provided a theoretical reference for the genetic improvement and marker-assisted breeding of a new strong gluten wheat variety Xinmai 45 at the molecular level.