不同浓度深色有隔内生真菌浸种对玉米幼苗生长的影响

Effects of Seed Soaking with Different Concentrations of Dark Septate Endophytic Fungi on Seedling Growth and Development of Plant

摘要: 本研究以深色有隔内生真菌(DSE)浸泡中糯1号玉米种子，探讨不同浓度DSE菌液浸种不同时间对其生长的影响。设置不同DSE浓度(20%、40%、60%、80%、100%),以无菌水和MMN培养液为对照组，测定植物株高、地上部干重，叶绿素SPAD值、根长和根系侵染率。结果表明，DSE菌液浸种能够显著提升玉米植株生长，促进叶片叶绿素的积累。其中，20%浓度浸种12 h的综合效果最显著，经济效益最佳。

Abstract: In this study, plant seeds were soaked with dark septate endophytic fungi（DSE） and the effects of different concentrations of DSE fungi solution soaking for different time on plant growth were discussed. The plant height, aboveground dry weight, chlorophyll SPAD value, root length and root infection rate were measured with different DSE concentrations of 20%, 40%, 60%, 80% and 100%, respectively, with sterile water and MMN medium as the control group. The results showed that seed soaking with DSE fungi solution could significantly improve the growth of corn plants and promote the accumulation of chlorophyll in leaves. Among them, the comprehensive effect of soaking seeds with 20% concentration for 12 h was the most significant and the economic benefit was the best.