Abstract: To clarify the genetic differences of the ten potato varieties at DNA molecular level like Nnongshu No.1（NNS-1）, Nnongshu No.2（NNS-2）, Nnongshu No.3（NNS-3）, Nnongshu No.4（NNS-4）, Nnongshu No.5（NNS-5）, Nnongshu No.6（NNS-6）, Red Color No.5（HC-5）, Purple color No.1（ZC-1）, purple color No.2（ZC-2） and purple color 3（ZC-3） and to extract genomic DNA of ten newly bred potato varieties of which genetic difference and cluster were analysed by SSR molecular marker technique. Ten pairs of suitable SSR primers were screened out, and 117 SSR polymorphism bands were obtained by PCR amplification of genomic DNA of 10 potato varieties, with the polymorphism ratio of 84.17%. Cluster analysis showed that the genetic distance（GD value） of 10 potato varieties varied from 0.1264 to 0.714 3. Based on the GD value of 0.55, the 10 materials were divided into