Abstract: In this experiment, the SEP gene of Cymbidium faberi was cloned, analyzed and expressed by real-time fluorescence quantitative analysis（RT-qPCR）. The results showed that the cloned gene was MADS gene, and the encoded protein had MADS conserved domain, which had the highest homology with Cymbidium geringii AXY 87448.1, reaching 98%. The gene was named as CfSEP1, and the accession number was MW 654191. RT-qPCR experiment analysis showed that the relative expression abundance of CfSEP1 in various tissues and organs of Cymbidium goeringii was as follows: petal（Ⅲ）＞sepal（Ⅲ）＞lip petal（Ⅲ）＞bud（Ⅱ）＞gynostemium（Ⅲ）＞ovary（Ⅲ）＞inflorescence rachis（Ⅱ）＞inflorescence rachis（Ⅲ）,showing highly abundance expression in flower organs,especially in tepals. It was not expressed in the roots and leaves of vegetative growth period, bud period and full-blossom period. The expression of CfSEP1 in the organs and tissues of Cymbidium goeringii increased gradually during the three growth stages, which indicated that CfSEP1 mainly regulated the growth and development of reproductive organs of Cymbidium faberi and promoted its reproductive growth.