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不同处理方式对沙枣种子萌发的影响研究

Study on Effects of Different Treatments on Seed Germination of Elaeagnus angustifolia L.

  • 摘要: 本试验通过培养温度、热激和赤霉素(GA3)3种处理方式探讨对沙枣种子萌发的影响。结果表明,培养温度显著影响沙枣种子萌发,其中20℃培养条件下种子发芽率最高、25℃环境下发芽势最高;低温下沙枣种子发芽高峰期推迟,发芽周期长;高温处理下沙枣种子发芽高峰期提前,发芽周期缩短,但发芽率低;30℃热激处理下发芽率最高,50℃热激处理下发芽势最高,60℃和70℃热激处理下,胚根发育最好;GA3浓度与时间的交互作用显著影响种子萌发,50 mg/L GA3浸泡2 d后,沙枣种子萌发效果最佳,发芽率为83.20%,发芽势为69.40%,50 mg/L GA3浸泡3 d处理下,根长最长。研究表明,25℃的培养条件下,50 mg/L的GA3处理沙枣种子2 d或30~50℃热激可促进沙枣种子快速萌发。

     

    Abstract: The effect of different treatments on seed germination of Elaeagnus angustifolia L. were studied from three aspects of culture temperature, heat shock treatment and gibberellin(GA3). The results showed that influence of culture temperature was extremely significant on seed germination, of which germination rate of seeds was the highest under 20 ℃ and germination potential was the highest under 25 ℃. Under low temperature, seed germination peak was delayed and the germination cycle was long, seed germination peak was advanced and the germination cycle was shortened under high temperature, but the germination rate was low.In response to the heat shock, germination rate of seeds at 30 ℃ was the highest, germination potential at 50 ℃ was the highest, The radicle development was best at 60 ℃ and 70 ℃.The interaction between GA3 concentration and time significantly affected seed germination. After soaking in 50 mg/L GA3 for 2 days, seed germination effect was the best, with germination rate of 83.20% and germination potential of 69.40%. Under soaking in 50 mg/L GA3 for 3 days, root length was the longest.Study showed that under the condition of 25 ℃, seeds were treated with 50 mg/L GA3 for 2 days or 30-50 ℃ heat shock, which could promote the rapid germination of seeds.

     

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