Abstract: Trichosanthes kirirowii Maxim has high edible and medicinal value, aiming at the current situation of serious shortage of high-quality seedling market supply, it is of great significance to study the rapid propagation technology system of Trichosanthes kirirowii Maxim.In this study, the high-quality and robust stem segments Wanlou 20 with axillary buds were selected as explants, using different sterilizing agents and sterilization methods, and adjusting the types of hormones and levels in each period.The technology and methods of asexual and rapid propagation by means of micro-cutting were studied.The results showed that the effect of the sterilization of Trichosanthes kirilowii maxim explants using 75% alcohol for 30 seconds + sterile water for 2-3 times + 0.1% HgCl₂ for 10 min + sterile water for 3-4 times was better, and the contamination rate was 26.3%.The average total contamination rate of alternate sterilization was 35.7%, and the average total contamination rate of a single sterilant was 71%.Therefore, a combination of alternate sterilization and multiple sterilization could be used to achieve the purpose of eliminating bacteria in practice.The establishment of the tissue culture system of Trichosanthes kirilowii maxim, the suitable medium for primary induction was MS + 6-BA 0.1 mg/L + NAA 0.05 mg/L,the axillary bud germination rate of stem segment was 79.4%;micropropagation medium was MS + 6-BA 0.6 mg/L + NAA 0.1 mg/L,the multiplication coefficient was 6.75; rooting medium was MS + NAA 0.6 mg/L + IBA0.6 mg/L,the rooting rate was 88.6%.The seedlings were transplanted into mixed earthworm soil and seedling substrate（1∶1）, and the survival rate was as high as 86.7%, laying a good foundation for realizing factory breeding of high-quality seedlings of Trichosanthes kirilowii maxim.