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基于SRAP分子标记分析彩色马铃薯品种间遗传关系

Genetic Diversity Research of Color Potato Cultivars Based on SRAP Molecular Markers

  • 摘要: 采用SRAP标记对收集的24份彩色马铃薯种质资源遗传多样性进行分析,探讨其遗传多样性及种质间亲缘关系,以期为彩色马铃薯种质资源高效利用及新品种选育奠定基础。结果表明,14对SRAP多态性引物共检测到74个多态性位点,多态性信息量(PIC)均值为0.87,有效等位基因数(Ne)均值为1.61,Nei’s遗传多样度(H)均值为0.36,Shannon信息指数(I)均值为0.54。24份彩色马铃薯种质间遗传相似系数介于0.45~0.78之间,均值为0.61,表明供试海雀稗种质间存在较大遗传差异。聚类分析显示,当GS=0.61时24份彩色马铃薯种质被划分为四大类群。主成分分析发现,Ⅲ、Ⅳ类群间有重叠现象,说明它们之间亲缘关系较近。24份彩色马铃薯种质具有较丰富的遗传多样性,可通过进一步研究应用于新品种选育。

     

    Abstract: The genetic diversity of the collected 24 colored potato germplasm resources were analyzed by SRAP molecular markers and the genetic relationships among the germplasm was discussed in order to provide a groundwork for the efficient utilization of colored potato germplasm resources and new varieties breeding. The results showed that 74 locis were detected by 14 pairs of SRAP polymorphic primers and the mean polymorphism information(PIC) was 0.87, the mean number of effective alleles(Ne) was 1.61, and the average Nei’s genetic diversity index(H)was 0.36, and the mean value of Shannon information index(I) was 0.54. The genetic similarity coefficients among the 24 colored potato germplasms ranged from 0.45 to 0.78 with an average value of 0.61, meaning there were large genetic differences among the tested puffin germplasms.Cluster analysis showed that when GS=0.61, the 24 colored potato germplasms were divided into four groups.Principal component analysis found that there was overlap between Group Ⅲ and Ⅳ, indicating that they were closely related.The 24 colored potato germplasms were abundant of genetic diversity, which could be applied to the selection of new varieties through further research.

     

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