Abstract: Passiflora edulis Sims are susceptible to pathogenic infestation after harvesting, bringing about water loss and crumpling, leading to rot and deterioration in serious cases, affecting its economic value. In order to extend the shelf life of Passiflora edulis Sims, this study isolated and purified the epidermal fungi from fresh fruits of Passiflora edulis Sims and analyzed the fungal composition. Confrontation tests were carried out using Trichoderma harzianum and Eurotium cristatum separately to observe the inhibition effect. Preparation of Trichoderma harzianum tea infusion, Eurotium cristatum tea infusions, mixed tea infusions of Trichoderma harzianum and Eurotium cristatum for post-harvest storage test of Passiflora edulis Sims. The results showed that nine genera of fungi were isolated from the fresh Passiflora edulis Sims, including one strain of Irpx, one strain of Strelitziana, two strains of Aspergillus, two strains of Trametes, two strains of Botryosphaeria, two strains of Epicoccum, two strains of Subulicystidium, two species of Cladosporium sp. with a total of three strains, and two species of Fusarium sp. with a total of eight strains. The confrontation test found that Trichoderma harzianum had the strongest inhibitory effect on Trametes hispida（TH） with 88.1% inhibition rate, and Eurotium cristatum had the strongest inhibitory effect on Irpex lacteus（LL） with 27.2% inhibition rate. The storage test revealed that the treatment effect of the Eurotium cristatum tea infusions was the best, with 0 pericarp shrinking in 0-4 day and only a few pericarp shrinking in the 5 th day and shrinkage rate of Passiflora edulis Sims was 1.17. With the increase of storage time, the shrinked area of the epidermis increased, and the shrinkage rate was 2 on the 7 th day, and all the wrinkles appeared on the 9 th day, and the shrinkage rate reached 2.33. When the fruit was cut open at the 14 th day, the flesh was intact and the juice was bright and odorless, and the shelf life of Passiflora edulis Sims was extended compared with the control group.