Abstract: In this study, high-throughput sequencing on the Illumina Hiseq platform was used to sequence, annotate, assemble and analyze the chloroplast whole genome of the medicinal plant Dendrobium candidum, and download 13 published orchid plants and 2 outgroups from NCBI for phylogenetic analysis. The results showed that the whole genome size was 153 508 bp, the large single copy region was 85 759 bp, the small single copy region was 14 973 bp, and the two inverted repeat regions were 26 419 bp; a total of 133 genes, 93 protein-coding genes, and 32 transfer RNA genes were annotated and 8 ribosomal rRNA genes, the content of GC accounted for 37.51% of the total number of bases; a total of 47 long repeats and 63 simple repeats were detected; the phylogenetic results were closest to Dendrobium nobile.