Abstract: High temperature has become one of the most important abiotic stress factors restricting maize production worldwide in recent years, but the molecular mechanism of maize resistance to high temperature stress is still very limited. In this study, two maize varieties Zhongdi 88（resistant to high temperature stress） and Xianyu 335（susceptive to temperature stress） were selected as experimental materials, in order to study the expression of genes related to zeatin synthesis pathway in pollen at 42 ℃ and 28 ℃. The results showed that five genes related to zeatin synthesis were identified, named Zmco5, Zmit4, Zmco4 b, Zmco12 and Zmczg1, with 582, 364, 534, 528 and 465 amino acids with gene-encoded protein, respectively. Bioinformatics analysis was performed on the expression proteins of the five genes, including common physical and chemical properties, functional sites, phosphorylation modification, secondary structure and tertiary structure, etc. The results showed that the biological phenotypes of different proteins were different. The results of gene expression demonstrated that the expression differences of these 5 genes in Zhongdi 88 were more than two times under the condition of normal temperature as control, the expression of Zmco5 gene was up-regulated and the other genes were down-regulated; while for Xianyu 335, the expression of Zmco5 and Zmczg1 were up-regulated, and the other three were down-regulated. These five genes were all involved in the zeatin synthesis pathway and were closely related to the biosynthesis of zeatin in cells.