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PEG模拟干旱胁迫对苘麻种子萌发的影响

Effects of PEG Simulated Drought Stress on Seed Germination of Abutilon theophrasti Medicus

  • 摘要: 为了探究干旱环境下苘麻种子的萌发情况,了解苘麻种子对干旱环境的抵抗能力,采用控制试验探究不同温度、光照及PEG-6000模拟干旱胁迫对苘麻种子萌发的影响,测定苘麻种子的发芽率、发芽势和发芽指数。结果表明,苘麻种子最适萌发条件为昼夜温度为35℃/25℃、自然光照(12 h光照、12 h黑暗),随着PEG-6000浓度升高,苘麻种子的萌发初始期和萌发高峰期也会相应延长;随着胁迫程度加深,种子发芽势和发芽指数均逐渐下降,但苘麻种子的日相对萌发率与累积发芽率在水势为-0.2 MPa时达到最大,分别为15%和53%;此时苘麻种子的发芽效果高于对照组(蒸馏水处理)。研究表明,苘麻种子对低浓度干旱胁迫具有一定抗性,但高浓度下苘麻种子萌发会停滞。

     

    Abstract: In order to explore the germination of Abutilon theophrasti Medicus seeds in arid environment and understand the resistance of Abutilon theophrasti Medicus seeds to drought environment. The effects of drought stress simulated by different temperature, light and polyethylene glycol on the germination of Abutilon theophrasti Medicus seeds were studied by control experiment, and the germination rate, germination potential and germination index of Abutilon theophrasti Medicus seeds were determined. The results showed that Abutilon theophrasti Medicus seeds were the most suitable for germination under the conditions of 35 ℃/25 ℃, natural light(light for 12 hours and darkness for 12 hours). With the increase of PEG-6000 concentration, the initial germination period and germination peak period of Abutilon theophrasti Medicus seeds would be prolonged accordingly. With the deepening of stress, the seed germination potential and germination index began to decrease gradually, but the daily relative germination rate and cumulative germination rate of Abutilon theophrasti Medicus seeds reached the maximum when the water potential was-0.2 MPa, reaching 15% and 53%, respectively. At this time, the germination effect of Abutilon theophrasti Medicus seeds was higher than that of the control group(distilled water treatment). Research indicated that Abutilon theophrasti Medicus seeds had certain drought resistance under low concentration drought stress, but the germination process of Abutilon theophrasti Medicus seeds stagnated at high concentration.

     

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