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基于KASP标记的国审小麦新品种长6990优异基因解析

Excellent Genes Analysis of a New State-certitied Wheat Variety Chang 6990 Based on Kompetitive Allele-specific PCR

  • 摘要: 以小麦新品种长6990为材料,利用中国农业科学院作物所何中虎老师课题组开发的59对包括适应性、籽粒大小、品质和抗逆性相关分子标记,运用高通量KASP分型标记技术对长6990全基因组进行检测。结果表明,适应性相关11个基因位点中,长6990含有7个优异等位变异,优异变异率为63.6%;籽粒相关10个基因位点中,长6990含有TaSus1-7B、TaSus1-7A、TaGS5-A1、GW2-6B等4个基因的优异等位变异,该优异位点的聚合可能是其表现丰产的重要原因;12个品质相关基因中,长6990含有5个优异等位变异,优异等位变异率为41.7%;在抗病性方面,长6990聚合了2个主效抗叶锈病基因(Lr14a、Lr46)和一个重要抗赤霉病基因(Fhb1),是其抗病性表现突出的原因;在抗逆相关6个基因位点中,检测到2个优异变异位点,为抗穗发芽位点PHS1和抗旱位点Dreb-B1,抗旱位点为微效位点,推测长6990突出的抗旱特性可能受其他位点的调控,为下一步抗逆基因挖掘指明了方向。

     

    Abstract: The new wheat variety Chang 6990 was used as the material, using 59 pairs of molecular markers, including adaptability, grain size, quality and stress resistance, developed by Professor He Zhonghu research group of Crop Sciences, Chinese Academy of Agricultural Sciences, high-throughput KASP typing technique was used to detect the whole genome of Chang 6990. The results showed that among the 11 genes related to adaptability, Chang 6990 contained 7 excellent allelic variations, and the excellent variation rate was 63.6%. Among the 10 loci related to grains, Chang 6990 contained excellent alleles of TaSus1-7B, TaSus1-7A, TAGS5-A1 and GW2-6B, and the aggregation of these excellent loci could be an important reason for its high yield. Among the 12 quality related genes, Chang 6990 contained 5 excellent allelic variations, and the excellent allelic variation rate was 41.7%. In terms of disease resistance, Chang 6990 aggregated two major leaf rust resistance genes(Lr14a, Lr46) and an important scab resistance gene(Fhb1), which was the reason for its outstanding disease resistance.Among the 6 gene loci related to stress resistance, 2 excellent mutation loci were detected, which were Phs1 for resistance to spike germination and Dreb-B1 for drought resistance, and the drought resistance loci was a slight effect loci. It was speculated that the outstanding drought resistance characteristics of Chang 6990 might be regulated by other loci, which pointed out the direction for the next step of stress resistance gene excavation.

     

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