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白鲜种子休眠解除过程中的转录组分析

Transcriptome Analysis of Dormancy Release in Dictamnus dasycarpus Turcz. Seeds

  • 摘要: 以解除休眠过程不同阶段的种子为材料,通过高通量测序技术对样品转录组进行测序分析。共获得135.98 Gb的原始数据,302 453条Unigenes,注释到七大功能数据库(GO、KEGG、KOG、NR、TrEMBL、Pfam、Swiss-Prot),分别有139 276(NR:46.05%)、128 449(TrEMBL:42.47%)、86 498(Swiss-Prot:28.60%)、90 564(KOG:29.94%)、103 481(KEGG:34.21%)、108 540(GO:35.89%)以及90 410(Pfam:29.89%)条Unigenes得到功能注释。通过GO分类和KEGG Pathway富集性分析,分别归于59个GO类别和145条代谢途径。同时,有6 081个Unigenes被注释为转录因子。利用高通量测序技术获得白鲜转录组信息,有助于从分子水平对白鲜进行深入研究。

     

    Abstract: The seeds in different stages of dormancy release process were taken as materials, and the transcriptome of the samples was sequenced and analyzed by high-throughput sequencing technology. 135.98 Gb of original data were generated, 302 453 Unigenes were annotated to 7 major functional databases(GO, KEGG, KOG, NR, TrEMBL, Pfam and Swiss-prot). 139 276(NR: 46.05%), 128 449(TrEMBL: 42.47%), 86 498(Swiss prot: 28.60%), 90 564(KOG:29.94%), 103 481(KEGG:34.21%), 108 540(GO:35.89%) and 90 410(Pfam: 29.89%) Unigenes were annotated respectively. By GO classification and KEGG pathway enrichment analysis, 59 GO categories and 145 metabolic pathways were assigned.Simultaneously, 6 081 Unigenes were annotated as transcription factors. Using high-throughput sequencing technology to obtain the transcriptome information of white fresh food was helpful to further study white fresh food at the molecular level.

     

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