Abstract: Superior dwarf source Shiai 2 and 09107 F₆ with high resistance to stripe rust and powdery mildew were hybridized, and the dwarf gene varieties, introduction rate, culm reduction effect and disease resistance in F₂ generation were detected by molecular markers. The results showed that three dwarf genes（Rht-B1b, Rht4 and Rht8） in 120 wheat lines of F₂ generation were detected, among which Rht-B1b gene accounted for 69.2%, Rht4 gene accounted for 78.3% and Rht8 gene accounted for 34.2%. The average wheat height was 56 cm, and a percentage of 34.1% in F₂ generation were resistant to powdery mildew and stripe rust. F₂ generation newly created had good resistance to disease and these available excellent wheat germplasm resources with 3 dwarf genes would provide important intermediate germplasm materials for the breeding and applications.