Abstract: Using the genome of wheat variety China Chun as reference sequence, the TaCYS family genes of wheat were identified by Hummer search technique at the whole genome level. The evolutionary relationship, gene structure, conserved base sequence and cis-acting elements of TaCYS family genes were analyzed. The results showed that through phylogenetic tree analysis, TaCYS were classified into three groups: A, B and C, and the gene structure and motif distribution of TaCYS in the same group were relatively conserved. Chromosomal mapping showed that TaCYS was localized on 19 chromosomes except chr 6 A and chr 5 B. By analyzing the cis-elements in the promoter region, it was speculated that TaCYS gene expression might be regulated by light, temperature, hormone and other conditions. Analysis of transcriptome data of wheat showed that most of TaCYS of class A were expressed in roots, leaves, ears and grains of wheat at different stages, while most of TaCYS of group B and C were expressed only in grains during reproductive development. TaCYS gene had specificity in resistance to different trophic fungi invasion. TaCYS35-D and TaCYS37-D could activate PTI response, and the response to biological stress was more intense.