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Study on the Infrared Spectrum Characteristics of Different Edible Beans

  • 摘要: 对不同食用豆的营养成分和特点建立一种简单、有效的检测方法。以8种不同食用豆类为试材,应用傅里叶变换红外光谱(FTIR)分析方法,通过比较各种豆类红外光谱特征和官能团特征峰的性质,鉴定比较了不同豆类中三大主要营养成分蛋白质、脂肪和碳水化合物红外光谱图吸收峰的位置、强度和峰型的差异,并对其所含营养成分中官能团和极性键振动进行结构分析。结果表明,不同食用豆类碳水化合物的吸收峰在3 292 cm-1附近OH伸缩振动和1 143 cm-1、1 051 cm-1附近的CO和COC伸缩振动;蛋白质的吸收峰显示在3 292 cm-1附近的NH伸缩振动和1 645 cm-1、1 548 cm-1附近的蛋白质酰胺Ⅰ带和Ⅱ带吸收区;脂类物质的吸收峰显示在2 926 cm-1附近的CH2伸缩振动和1 384 cm-1附近的CH3弯曲振动。8种食用豆类营养成分都富含碳水化合物、蛋白质和脂肪类物质,但在官能团区有一定的差异,采用傅里叶变换红外光谱法能够直观、简便地比较不同食用豆类的营养特点。

     

    Abstract: A simple and effective method for detecting the nutritional components and characteristics of different edible beans was established. Eight different kinds of edible beans selected as test materials, and Fourier transform infrared spectroscopy(FTIR) analysis method was used to compare the infrared spectral characteristics of various beans and the properties of the characteristic peaks of functional groups. The identification and comparison of the position, intensity and peak shape of the absorption peaks of the three main nutrient components protein, fat, and carbohydrates in different beans were conducted. The structural analysis of the functional groups and polar bond vibrations in the nutrients contained in it was performed. The results showed that the absorption peaks of different edible legume carbohydrates were around 3 292 cm-1OHstretching vibration and 1 143 cm-1, 1 051 cm-1 nearCOandCOCstretching vibration. The absorption peak of protein showed theNHstretching vibration near 3 292 cm-1 and the absorption zone of protein amide band I and band II near 1 645 cm-1 and 1 548 cm-1. The absorption peak of lipids showed the CH2 stretching vibration near 2 926 cm-1 and the CH3 bending vibration near 1 384 cm-1. The 8 kinds of beans were rich in carbohy drates, proteins and lipids in their nutritional components, and their infrared spectrograms had certain differences in the functional group area, which showed that the application of infrared spectroscopy could quickly and intuitively compare the nutritional characteristics of different edible beans.

     

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