Abstract: In order to investigate the effects of CaCl₂ stress on seed germination and seedling growth of Periploca forrestii Schltr., stress experiments were carried out on seeds and seedlings of Periploca forrestii Schltr. with different concentration gradients of CaCl₂ solution, and seed-related germination indexes and seedling growth indexes after seed germination were analyzed under different concentrations of calcium stress. To compare the difference of seed germination characteristics under different concentrations of calcium stress, and the effects of calcium stress on the growth and physiological indexes of Periploca forrestii Schltr. seedlings. The results showed that low concentration calcium stress treatment could promote seed germination, while high calcium treatment inhibited seed germination. The germination potential, germination rate, germination index, daily germination rate and growth index decreased with the increase of calcium concentration. High calcium environment inhibited seed germination. With the increase of calcium stress concentration, the growth of the apparent traits（number of lateral branches, plant height） of the seedlings was gradually inhibited, and the chlorophyll content of the seedlings decreased. High concentration of calcium stress inhibited the growth of the seedlings and the photosynthesis of the leaves. The content of soluble sugar in the seedlings increased gradually with the increase of calcium concentration, while the activities of peroxidase, superoxide dismutase, glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase increased gradually, indicating that the seedlings were subjected to osmotic stress under calcium stress, and the seedlings mainly depended on the antioxidant oxidase system to remove oxygen free radicals during the growth process. Metabolic reaction by transamination. The comprehensive analysis showed that high calcium concentration could inhibit seed germination and seedling growth of Periploca forrestii Schltr.. Therefore, calcium concentration should be properly controlled in the future introduction and domestication process.