高级检索+

特种稻种质资源耐盐性综合评价及耐盐种质筛选

Comprehensive Evaluation on Salt Tolerance of Different Special Rice Germplasm Resources and the Salt-tolerant Germplasm Screening

  • 摘要: 以36种不同特种稻种质资源为试验材料,采用室内水培,分析盐胁迫对不同特种稻幼苗生物量和根系形态参数的影响。结果表明,通过主成分分析将11个测定指标转换为3个主成分,累积贡献率为71.43%,通过隶属函数值和权重分析进一步将3个主成分值简化成评价不同特种稻种质资源苗期耐盐性的综合评价值D,筛选到山稻、Y-9(黄白)、浙白和黑宝糯等4个苗期综合耐盐性强的特种稻种质资源。根据D值利用聚类分析将36份不同特种稻种质资源分为五类,第Ⅰ类群仅包含1份高耐盐种质,为山稻;第Ⅱ类群主要为高度盐敏感种质;第Ⅲ类群主要为盐敏感种质;第Ⅳ类群主要为弱耐盐种质;第Ⅴ类群主要为耐盐种质。结合逐步回归分析,得到D值与11个测定指标的最优回归方程:D=0.088+0.169×XRS+0.144×GRS+0.117×XRR+0.048×GRR-0.192×DRA。综上所述,相对地上部鲜重、相对地上部干重、相对根系鲜重、相对根系干重、相对根平均直径可作为特种稻种质资源苗期耐盐性鉴定的重要指标。幼苗期耐盐性较强的特种稻种质资源分别为山稻、Y-9(黄白)、浙白和黑宝糯。

     

    Abstract: 36 special rice germplasm resources used as experimental materials, the effects of salt stress on the seedling biomass and root morphological parameters of different special rice varieties were analysed using the indoor hydroponic method. The results showed that 11 determination indexes were simplified into three major components, with a cumulative contribution rate of 71.43%, using principal component analysis(PCA). With membership functions and index weight method, the three principal component values were further reduced to a comprehensive evaluation D value, for assessing the salt tolerance of different special rice germplasm resources at seedling stage. Four rice germplasms(Shandao, Y-9(Huangbai), Zhebai and Heibaonuo) were selected. According to the D value, cluster analysis assigned 36 rice germplasm resources into five groups. The group-Ⅰ contained only one high salt-tolerant genotype(Shandao); High salt-sensitive germplasm accessions were mainly found in the group-Ⅱ; The germplasm accessions showing salt-sensitive were resided in the group-Ⅲ; The group-Ⅳ was largely consisted of weak salt-tolerant germplasm; The germplasm genotypes with salt-tolerant were mainly clustered in group-Ⅴ. Combined with stepwise regression analysis, the optimal regression equation of 11 measurement indicators and D-value of was obtained: D=0.088+0.169×XRS+0.144×GRS+0.117×XRR+0.048×GRR-0.192×DRA. In conclusion, relative shoot fresh weight, relative shoot dry weight, relative root fresh weight, relative root dry weight and relative average root diameter were deployed as important indicators to identify salt-tolerant special rice germplasm resources at seedling stage. The selected germplasm resources of special rice with strong salt tolerance at seedling stage were Shandao, Y-9(Huangbai), Zhebai and Heibaonuo.

     

/

返回文章
返回