Abstract: The effects of different plant growth regulators on seed embryo germination, stem segment proliferation and seedling rooting were studied in order to establish tissue culture rapid breeding technology system of Sorbus hupehensisc. K. Schneid.. The results showed that the seed embryos of Sorbus hupehensisc. K. Schneid. were in MS+0.5 mg/L 6-BA+0.05 mg/L IBA+30 g/L sucrose+7 g/L carrageenan germination and normal growth, of which the average germination rate was 95%, much higher than the average induction rate of 5% without hormone medium combination, induced normal growth of embryos in the medium, 30 days after the germination of multiple true leaves and axillary buds. Stem segments with buds were transferred to MS+0.25 mg/L 6-BA+0.04 mg/L IBA+30 g/L sucrose+7 g/L carrageenan for 30 days, and 2.08 buds were induced per stem segment, with a proliferation rate of 490.5%. If stem segments with buds were transferred to MS+0.5 mg/L 6-BA+0.04 mg/L IBA+30 g/L sucrose+7 g/L carrageenan for 30 days, 4.1 buds were induced per stem segment on average, and the proliferation rate was 291%. The seedlings were cultured in MS+0.5 mg/L NAA+30 g/L sucrose+7 g/L carrageenan for 30 days, and the rooting rate reached 84%. Adventitic roots were induced at the base of the sprouts by callus regeneration, and an average of 4.41 roots were induced per sprout.