Abstract: A new generation of high-throughput, low-cost seed phenotype platform SeedGerm V 2.0 improved by adding spray device, which could be run unattended. Based on this seed phenotype platform, the seed germination kinetics of the local mustard cultivar ’Shuidong Sweet Mustard’ under different concentrations of NaCl stress were analyzed. The results showed that the seed platform could automatically collect and analyze the germination data of mustard seeds, which could be used in the study of salt stress. Lower NaCl concentration could promote the germination rate of mustard seeds, but with the increase of salt concentration, the germination rate of mustard seeds decreased rapidly, and the germination basically stopped when the concentration reached 0.300 mol/L, indicating that mustard seeds had strong ability of salt tolerance. The halo halothermal time（HaloTT） model parameters of Shuidong sweet mustard seeds were estimated as 2.26 mol/（L·h） for θ_halo, 0.22 mol/L for NaCl_b（50）, and 0.067 mol/L for the standard variance δ_NaClb. Then a prediction equation obtained from above paramaters, Probit（g） =（NaCl+2.26/t_g-0.22）/0.067, was developed, which could accurately predict the germination timing and rate of mustard seeds under different salt concentrations, and can be of great reference to breeding and producing for mustard.