Abstract: Nanyang, as an important overwintering region, wheat stripe rust epidemic, the resistance expression and gene distribution of stripe rust resistance in wheat varieties（lines） of Nanyang wheat area were understood in order to provide valuable recommendations for safe production in main wheat region, cultivar distribution and stripe rust resistance breeding. 22 major wheat varieties and 146 generation of high lines from Nanyang wheat area were taken as materials by using the mixed strains of popular races CYR32, CYR33 and CYR34 and natural occurrence. In addition, these materials were natural identification in Nanyang, artificial inoculation identification in Yangling, related molecular markers for detection of Yr2, Yr10, Yr18 and Yr32. The results showed that among among the 22 major wheat varieties, by natural identification, 12 showed moderate resistance or above, 4 showed moderate resistance to moderate susceptibility, and 6 showed moderate susceptibility or above. Besides, 14 were moderately resistant or above, and 8 were moderate susceptibility or above by artificial inoculation identification. Resistance genes Yr2, Yr10, Yr18, Yr32 were postulated in 21, 15, 0 and 4 materials, respectively. Yr gene combinations Yr2+Yr10, Yr2+Yr32, Yr2+Yr10+Yr32, were also postulated in 11, 1 and 3 materials, respectively. Among the 146 generation of high lines, 126 showed moderate resistance or above, 18 showed moderately susceptible and 2 showed susceptible. Moreover, 99 were moderately resistant or above, 20 showed moderately susceptible and 27 showed susceptible by artificial inoculation identification. Resistance genes Yr2, Yr10, Yr18, Yr32 were postulated in 141, 87, 0 and 35 materials, respectively. Yr gene combinations Yr2+Yr10, Yr2+Yr32, Yr10+Yr32, Yr2+Yr10+Yr32, were also postulated in 61, 8, 4 and 22 materials, respectively. The results showed that the major wheat cultivars like Xinong979, Zhengmai9023, Lankao198 and Bainong207 lost their resistance to stripe rust. Zhengmai7698, Nanyang996 and Xinong501 showed better rust resistance. Owning Yr2+Yr10+Yr32, Xinong511, Aikang58 and Xinong509 had great value in promotion and utilization. In total, the rate of Yr2+Yr10+Yr32, Yr32 was low in the major cultivars and the generation of high lines. Based on Nanyang wheat area, the utilization of Yr32 and stripe rust resistance gene polymerized varieties（lines） should be strengthened in promoting varieties, new variety selection and parental selection.