Abstract: The material used in this study was the 210 days capsules produced from pollinating the self-incompatible Paphiopedilum purpuratum. The effects of different basic culture media and additives on seedling aseptic germination and protocorm growth were studied. Results showed that the seeds were spindle-shaped and most of the embryos were poorly developed. The optimal basic culture medium for aseptic seed germination was found to be 1/4 MS, while the addition of coconut milk and banana juice significantly improved the germination rate. The best overall medium for germination and growth was found to be 1/4 MS+20 g/L sucrose+7 g/L agar+100 mL/L coconut milk+0.2 mg/L 6-BA+0.1 mg/L NAA, followed by 1/4 MS+20 g/L sucrose+7 g/L agar+100 mg/L banana puree+0.2 mg/L 6-BA+0.1 mg/L NAA. The former had a faster germination time and growth rate but no significant proliferation effect, while the latter had slower germination and growth rate but obvious tuber proliferation and differentiation. Adding a small amount of plant hormones to the germination culture medium could induce protocorm differentiation before transferring to a new container, which helped to avoid browning phenomenon. Using a 1∶1 mixture of humus soil and pine bark as the cultivation substrate, the survival rate of tissue-cultured seedlings after transfer planting exceeded 90%.