Abstract: In this experiment, the mature fruits of Solanum aculeatissimum were used as materials to explored the effects of different pretreatments on seed germination and the effects of different concentrations of S-attractants on S.aculeatissimum seedlings. The germination rate, germination index, POD activity, CAT activity, seedling height, stem diameter and other indicators were measured. The results showed that in the seed germination pretreatment experiment, wet and dry treatment for two times（A2） could significantly improved the seed germination ability and vitality, and improved the morphological and physiological indexes during seed germination. After the treatment with 2.0 ml/L S-inducer（S200）, the seedling height, stem diameter, taproot length, taproot diameter, POD activity, CAT activity and root activity of S.aculeatissimum reached the maximum value. Study showed that this treatment method could effectively promoted the growth and development of S.aculeatissimum seedlings.