Abstract: To understand the composition of functional genes for important agronomic traits in wheat breeding materials, 107 breeding parents were detected using 50 KASP markers of functional gene. The results showed that the proportion of superior alleles for dwarf genes Rht-B1 and Rht-D1 were 54.21% and 71.03%, respectively. The proportion of 1BL/1RS translocation line materials accounted for 41.12%, and had larger difference among different regional germplasms. The proportion of superior alleles in TaSus-7A, TaGW2-6B, and TaTGW6-4A gene were high for seed traits. The germplasm in Shaanxi contained more superior alleles of TaSus1-7B, TaSus2-2A, TaSus2-2B, TaGS-D1, TaGW5-A1, while the proportion of other regional breeding germplasm containing TaCwi-A1, TaGASR7-A1, TaGW2-6A superior alleles were higher. The alleles of resistant pre-harvest sprouting genes had not large difference in differed regional germplasms. The TaPHS1 was the main resistant gene and the proportion of superior alleles was 82.24%. The drought resistance genes CWI-4A, CWI-5D and TaSST-A2 had a high proportion in the tested materials, and the proportion of TaMoc-A1, TaSST-D1, Dreb-B1 and 1-feh-w3 had large difference in different regional breeding germplasms. The proportion of the superior alleles of disease resistance genes were low, while the proportion of the superior allele in Tsn1 was high in the materials. The proportion superior alleles associated with vernalization and photoperiod were high, and had two germplasms with three earliness genes. In this study, the functional genes of breeding parents identified favorable alleles in important agronomic traits, and provided reference for parental selection and molecular marker-assisted selection for wheat breeding.