Abstract: DNA methylation is a highly conserved epigenetic modification concerning many biology processes. In plants, cytosine DNA methyltransferase genes have been identified in several plant species. In this study, DNA methyltransferase genes in Deyeuxia angustifolia Kom. was identified and analysed on a genome-wide level. 29 DNA methyltransferases were identified in Deyeuxia angustifolia Kom.. They were divided into four subfamilies, DaMET, DaCMT, DaDRM and DaDNMT2 respectively. Based on the analysis of the conserved structural domains of these genes, DNA methyltransferases gene loss and replication events occurred during the evolutionary process. In addition, multiple cis-acting elements were identified in DNA methyltransferases, including light response, phytohormone response, stress response and plant growth and development-related elements. Besides, the transcriptional abundance of DNA methyltransferases under different habitat（typical meadow, swamp, and marshy meadow） conditions was studied. Most DaMET expression levels were significantly down-regulated in different habitats, while transcript abundance of both DaDRM genes were significantly induced.