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姜黄属植物叶绿体基因组特征研究

Studies on the Chloroplast Genome Features of Curcuma Species

  • 摘要: 以8株来源不同的姜黄属植物(3株姜黄,1株莪术,3株郁金和1株川郁金)为实验材料,基于Illumina测序平台,Get-Organelle软件组装出8个植物叶绿体基因组,结合Genbank中下载姜黄属植物叶绿体基因组33条,共得到41个姜黄属叶绿体基因组。姜黄属叶绿体基因组均为四分体结构,序列长度为159 423~163 539 bp, GC含量范围36.2%~36.3%,编码基因为125~133个;姜黄、蓬莪术、郁金和川郁金均含有133个基因,包括38个tRNA、8个rRNA和87个编码蛋白基因;检测到的SSR数量范围为97~128个,散在重复序列均为50个,基因组序列具有丰富的AT含量。基于整个叶绿体全基因组作为条形码构建的系统进化树显示姜黄属不是单系,不过药典中收录的五种姜黄属药材郁金、广西莪术、姜黄、蓬莪术、温郁金与其他姜黄属物种形成一个独立分支,提示这些药用姜黄属植物有非常密切的关系。但由叶绿体基因组建的系统树中一部分没有区分开来的姜黄属物种,有可能是姜黄属通过外形很难鉴定而导致的不准确性或者物种间相互杂交及渐近渗透等原因,需要结合核基因或其他技术手段做进一步鉴定。

     

    Abstract: Using 8 plants from different sources of Curcuma(3 Curcuma longa L.,1 Curcuma phaeocaulis,3 Curcuma aromatica Salisb. and 1 Curcuma sichuanensis X.)as test materials, eight plant chloroplast genomes were assembled based on Illumina sequencing platform and Get-Organelle software. Thirty-three turmeric chloroplast genomes were downloaded from Genbank, and 41 Curcuma chloroplast genomes were obtained. The chloroplast genome of Curcuma was tetrad structure, the sequence length was from 159 423 to 163 539 bp, the GC content was from 36.2% to 36.3%, and the coding genes were from 125 to 133. There were 133 genes in Curcuma longa L., Curcuma phaeocaulis, Curcuma aromatica Salisb. and Curcuma sichuanensis X., including 38 tRNA, 8 rRNA and 87 protein coding genes. The number of SSR detected ranged from 97 to 128, with 50 scattered repeats, and the genome sequence had abundant AT content. The phylogenetic tree based on the whole chloroplast genome as a bar code showed that Curcuma was not monophyletic, but the five Curcuma herbs such as Curcuma aromatica Salisb., Curcuma kwangsiensis S.G.Lee et C.F.Liang, Curcuma longa L., Curcuma phaeocaulis, Curcuma wenyujin Y. H. Chen et C. Ling, included in the pharmacopoeia, formed an independent branch with other Curcuma species, suggesting that these medicinal Curcuma plants were very closely related. However, some of the undistinguished Curcuma species in the phylogenetic tree composed of chloroplast genes could be due to the inaccuracy caused by the difficult identification of Curcuma by shape, or due to cross-breeding and asymptotic penetration among species, and further identification by nuclear genes or other technical means was needed.

     

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