Abstract: Different concentrations of plant growth regulators（brassinosterol（EBR）, indole acetic acid（IAA）, 6-benzylaminopurine（6-BA）, gibberellin（GA₃）） were used to treat Allium mongolicum Regel seeds with 4 storage years（0,4,8,10 years） to explore the effects of different plant growth regulators on seed germination of Allium mongolicum Regel. The results showed that compared with the control, 30 mg/L IAA treatment could break the dormancy of the seeds（stored for 0 years）, and the germination rate was 23.23%, 2.6 times that of the control. The germination rate of the seeds stored for 4 years under 15 mg/L IAA treatment was up to 84.85%, which was 1.1 times that of the control. The germination rate of the seeds stored for 8 years under 0.4 mg/L EBR treatment was 66.67%, which was 1.2 times that of the control. The germination rate of the seeds stored for 10 years after 0.1 mg/L 6-BA treatment was 12.12%, which was 4 times that of the control. The germination conditions of the seeds treated with the same plant growth regulator for different storage years were also different. Compared with the seeds with strong germination（storage for 4 years）, the lower concentration of plant growth regulator had obvious promotion effect on the germination and growth index of the seeds, and the higher concentration of plant growth regulator was better than the control in aspect of aging（storage for 10 years）.