Abstract: In order to understand the molecular mechanism of the effect of medium wave ultraviolet（UV-B） on the activity of waxy maize seeds, waxy maize inbred line N51 seeds were treated with UV-B irradiation at 0 min（control）, 30 min, 1 hours, 2 hours, 4 hours and 8 hours, and the activity indexes and transcriptomics were analyzed.The results showed that there was no significant difference between waxy maize inbred line N51 and the control.In the range of 0 min, 30 min, 1 hours, 2 hours, 4 hours and 8 hours, the germination potential, germination index and vitality index all showed a trend of first increasing and then decreasing, and the treatment length reached the highest at 1 hours. According to various vitality indexes, UV-B irradiation at 1 hours had the most significant effect on promoting corn seed germination.According to 0 min（control）, 1 hours, 2 hours 3 treatments, the seed vitality indexes of waxy maize showed a trend of first increasing and then decreasing. Transcriptome sequencing analysis screened out 5 differentially expressed genes that were first up-regulated and then down-regulated, and 869 differentially expressed genes that were first down-regulated and then up-regulated.GO annotation classification showed that UV-B treatment mainly affected the differential expression of germination-related phenotypes of waxy maize inbreeding line N51 seeds by affecting thylakoids, photosynthetic system light, cooperative use, photosynthetic membrane, photosynthetic antenna protein, metabolic pathway, nitrogen metabolic pathway and other metabolic pathways.The enrichment of KEGG pathway included phenylpropanoid biosynthesis, flavonoid biosynthesis, starch and sucrose metabolism.