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基于SSR分子标记的甜瓜杂交种子纯度及真实性鉴定

Purity and Authenticity Identification of Cucumis melo L. Hybrid Seeds Based on SSR Molecular Markers

    摘要
  • 摘要: 本研究以广泛种植的甜瓜杂交种‘金蜜6号’和杂交新品种‘南疆蜜1号’及其亲本为试验材料，采用简单重复序列分子标记(SSR)检测技术，选取18对甜瓜多态性丰富的SSR核心引物进行纯度和真实性鉴定。结果表明，‘金蜜六号’和‘南疆蜜1号’在18对SSR核心引物筛选中均获得多对特异性引物，表现出多态性条带，可以用来鉴定种子纯度，‘金蜜六号’种子的纯度为97.87%,特异性引物：SSR12833(A)、CMBR052(C)、ECM147(I)、CMBR002(K)、CM26(L)、MU9175-1(M)、MU5554-1(N)、SSR00398(O)、TJ10(Q)、CMBR154(R),其中，编号C、I、K、N、O引物条带清晰，特异性强；‘南疆蜜1号’的种子纯度为97.56%,特异性引物：SR12833(A)、CMBR097(F)、MU4104-1(G)、NR38(H)、ECM147(I)、CMBR002(K)、MU9175-1(M)、MU5554-1(N)、CMBR154(R),其中，编号F、G、H、I、N、R引物条带清晰，特异性强。SSR分子标记鉴定结果与田间鉴定结果一致。SSR分子标记方法速度快、时间短，鉴定结果准确，可为室内开展杂交种‘金蜜六号’和‘南疆蜜1号’的纯度鉴定工作提供技术保障。

     

    Abstract: This study used the widely planted Cucumis melo L. hybrid ’Jinmi6’ and the new hybrid variety ’Nanjiangmi1’ and their parents as experimental materials. Using SSR（Simple repeat sequence molecular marker） detection technology, 18 pairs of SSR core primers with rich polymorphism in Cucumis melo L. were selected for purity and authenticity identification. The results showed that ’Jinmi6’ and ’Nanjiangmi1’ obtained multiple pairs of specific primers in 18 pairs of SSR core primer screening, exhibiting polymorphic bands that could be used to identify seed purity. The purity of ’Jinmi6’ seeds was 97.87%, with specific primers including SSR12833（A）, CMBR052（C）, ECM147（I）, CMBR002（K）, CM26（L）, MU9175-1（M）, MU5554-1（N）, SSR00398（O）, TJ10（Q）, CMBR154（R）, of which there were clear bands and strong specificity for primers C, I, K, N, O. The seed purity of ’Nanjiangmi 1’ was 97.56%, with specific primers such as SR12833（A）, CMBR097（F）, MU4104-1（G）, NR38（H）, ECM147（I）, CMBR002（K）, MU9175-1（M）, MU5554-1（N）, and CMBR154（R）. Primers numbered F, G, H, I, N, and R had clear bands and strong specificity. The results of SSR molecular marker identification were consistent with those of field identification.

     

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