Abstract:
SVP(Short vegetative pase) transcription factor plays an important role in regulating plant growth and development and responding to abiotic stress. In this study, an SVP-like gene was obtained from Blumea balsamifera by homologous cloning and RACE techniques, which was named as BbSVP. Bioinformatics analysis showed that the coding region of the BbSVP gene was 681 bp long and encoded 226 amino acids, belonging to the MADS-box protein family, and there were no obvious signal peptides and transmembrane domains in the protein. Homology comparison showed that BbSVP was closely related to HaSVP X1 of sunflower, which was also in the asteraceae family. Further studies showed that BbSVP was concentrated in the nucleus. BbSVP gene had tissue specific expression, and the expression level of BbSVP gene was highest in leaves and lowest in flowers. After abiotic stress treatment on Blumea balsamifera,RT-qPCR analysis showed that BbSVP could respond to PEG, NaCl and 4 ℃ low temperature stress at the transcriptional level. The results show that BbSVP gene was conserved in sequence and function, and it could be involved in regulating the response to abiotic stress.