兰属植物珍珠矮试管开花技术研究

付双彬; 杨燕萍; 应震; 高绪勇; 周庄

doi:10.16590/j.cnki.1001-4705.2024.07.136

兰属植物珍珠矮试管开花技术研究

浙江省农业科学院/浙江省亚热带作物研究所

基金项目:

浙江省农业科学院专项项目(2022R26CB002)

浙江省农业科学院瓯海科创中心项目(2021OHKC0005)

温州市科学技术协会项目(kjfw04)

温州农业新品种选育协作组项目(2019ZX004-3)

详细信息

作者简介:
付双彬(1989—),男(汉族),河北唐山人,硕士,助理研究员,主要从事兰花组织培养研究(E-mail:shuangbinfu@gmail.com)

通讯作者:
周庄(1978—),男(汉族),浙江温州人,硕士,副研究员,主要从事植物分类学与基因组学研究(E-mail:zhuangchow@126.com)

中图分类号: S682.31
计量
- 文章访问数: 3
- HTML全文浏览量: 0
- PDF下载量: 0
出版历程
- 收稿日期: 2024-02-23

Study on in Vitro Flowering Technology of Cymbidium Plant Cymbidium nanulum

Zhejiang Academy of Agricultural Sciences, Zhejiang Institute of Subtropical Crops

摘要

摘要: 为了保护和开发利用兰花新种珍珠矮，加快育种进程，本研究通过根状茎直接和再生植株间接的方式来诱导试管开花。结果表明，在含有1 mg/L 6-BA,30 g/L蔗糖以及2 g/L蛋白胨的调整MS培养基（165 mg/L NH₄NO₃、380 mg/L KNO₃和850 mg/L KH₂PO₄）上直接诱导根状茎获得的正常试管花数量最高，平均为2.6个。含有3 mg/L 6-BA、1 mg/L NAA和100 mL/L椰汁的MS或1/2 MS培养基最适宜珍珠矮根状茎再生；在含有3 mg/L 6-BA和20 g/L蔗糖的MS培养基间接诱导再生植株获得试管花数量最高，平均为0.8个。
- 兰花 /
- 珍珠矮 /
- 根状茎 /
- 再生植株 /
- 试管开花
Abstract: In order to protect, develop and utilize the new Cymbidium plant Cymbidium nanulum and accelerate the breeding process, this study induced in vitro flowering by direct rhizomes and indirect regenerated plants. The results showed that the highest number of normal flowers were obtained by direct induction on the adjusted MS medium containing 1 mg/L 6-BA, 30 g/L sucrose and 2 g/L peptone（165 mg/L NH₄NO₃, 380 mg/L KNO₃ and 850 mg/L KH₂PO₄）, with an average of 2.6. MS or 1/2 MS medium containing 3 mg/L 6-BA, 1 mg/L NAA and 100 mL/L coconut water was the most suitable medium for regeneration of Cymbidium nanulum rhizomes. In MS medium containing 3 mg/L 6-BA and 20 g/L sucrose, the number of flowers obtained by indirect induction was the highest, with an average of 0.8.
- orchid /
- Cymbidium nanulum /
- rhizomes /
- regenerated seedings /
- in vitro flowering

HTML全文

参考文献(39)

[1]	Lee Y I,Yeung C T.Orchid propagation from laboratories to greenhouses—methods and protocols[M].Humana press,2018.
[2]	吴应祥,陈心启.中国兰属两新种[J].植物分类学报,1991,29(6):549-552.
[3]	和寿星,薛润光,李兆光,等.兰属新种珍珠矮的组织培养与植株再生[J].植物生理学通讯,2007,43(3):517-518.
[4]	Teixeira da Silva J A,Kerbauy G B,Zeng S J,et al.In vitro flowering of orchids[J].Critical Reviews in Biotechnology,2014,34(1):56-76.
[5]	陈达菊,曾瑞珍,杨青,等.建兰与寒兰杂交后代试管花诱导及其形成过程的形态学及细胞学观察[J].热带作物学报,2015,36(10):1839-1844.
[6]	Duan J X,Yazawa S.In vitro floral development in×Doriella Tiny (Doritis pulcherrima×Kingiella philippinensis)[J].Scientia Horticulturae,1994,59(3-4):253-264.
[7]	Chang C,Chang W C.Cytokinins promotion of flowering in Cymbidium ensifolium var.misericors In vitro[J].Plant Growth Regulation,2003,39(3):217-221.
[8]	Sim G E,Loh C S,Goh C J.High frequency early in vitro flowering of Dendrobium madame thong-in (Orchidaceae)[J].Plant Cell Reports,2007,26(4):383-393.
[9]	Sim G E,Goh C J,Loh C S.Induction of in vitro flowering in Dendrobium madame thong-in (Orchidaceae) seedlings is associated with increase in endogenous N6-(Δ2-isopentenyl)-adenine (iP) and N6-(Δ2-isopentenyl)-adenosine (iPA) levels[J].Plant Cell Reports,2008,27(8):1281-1289.
[10]	Rahman T K B M M.In vitro seed germination and flowering of Dendrobium palpebrae Lindl.Orchid[J].Journal of Global Biosciences,2017,6(1):4748-4757.
[11]	Khasim S M,Hegde S N,González-Arnao M T,et al.Orchid Biology:Recent Trends & Challenges[M].Singapore:Springer Nature Singapore Pte Ltd,2020.
[12]	Murashige T,Skoog F.A revised medium for rapid growth and bio assays with tobacco tissue cultures[J].Physiologia plantarum,1962,15:473-494.
[13]	Deb C R,Sungkumlong.Rapid multiplication and induction of early In vitro flowering in Dendrobium primulinum Lindl[J].Journal of Plant Biochemistry and Biotechnology,2009,18(2):241-244.
[14]	Kostenyuk I,Oh B J,So I S.Induction of early flowering in Cymbidium niveo-marginatum Mak in vitro[J].Plant Cell Reports,1999,19(1):1-5.
[15]	Kachonpadungkitti Y,Romchatngoen S,Hasegawa K,et al.Efficient flower induction from cultured buckwheat (Fagopyrum esculentum L.) node segments In vitro[J].Plant Growth Regulation,2001,35(1):37-45.
[16]	Franklin G,Pius P K,Ignacimuthu S.Factors affecting In vitro flowering and fruiting of green pea (Pisum sativum L.)[J].Euphytica,2000,115(1):65-74.
[17]	Ferreira W M,Suzuki R M,Pescador R,et al.Propagation,growth,and carbohydrates of Dendrobium second love (Orchidaceae) In vitro as affected by sucrose,light,and dark[J].In Vitro Cellular & Developmental Biology-Plant,2011,47(3):420-427.
[18]	Zeng S J,Liang S,Zhang Y Y,et al.In vitro flowering red miniature rose[J].Biologia Plantarum,2013,57(3):401-409.
[19]	Ziv M,Naor V.Flowering of geophytes In vitro[J].Propagation of Ornamental Plants,2006,6(1):3-16.
[20]	Tee C S,Maziah M,Tan C S.Induction of In vitro flowering in the orchid Dendrobium sonia 17[J].Biologia Plantarum,2008,52(4):723-726.
[21]	Kerbauy G B.In vitro flowering of Oncidium varicosum mericlones (orchidaceae)[J].Plant Science Letters,1984,35(1):73-75.
[22]	Hirst P M,Ferree D C.Rootstock effects on the flowering of 'Delicious' apple.Ⅱ.nutritional effects with specific reference to phosphorus[J].Journal of the American Society for Horticultural Science,1995,120(6):1018-1024.
[23]	Teixeira Da Silva J A,Yam T,Fukai S,et al.Establishment of optimum nutrient media for In vitro propagation of Cymbidium Sw.(Orchidaceae) using protocorm-like body segments[J].Prop.Ornamental Plants,2005,5(3):129-136.
[24]	Duan J X,Yazawa S.Floral induction and development in Phalaenopsis In vitro[J].Plant Cell,Tissue and Organ Culture,1995,43(1):71-74.
[25]	Lunn J E,MacRae E.New complexities in the synthesis of sucrose[J].Current Opinion in Plant Biology,2003,6(3):208-214.
[26]	Bernier G,Périlleux C.A physiological overview of the genetics of flowering time control[J].Plant Biotechnology Journal,2005,3(1):3-16.
[27]	Bernier G,Corbesier L,Périlleux C.The flowering process:on the track of controlling factors in Sinapis alba[J].Russian Journal of Plant Physiology,2002,49(4):445-450.
[28]	George E F,Hall M A,Klerk G J D.The components of plant tissue culture media Ⅱ:organic additions,osmotic and pH effects,and support systems[M]//Plant Propagation by Tissue Culture:Volume 1.The Background.Dordrecht:Springer Netherlands,2008:115-173.
[29]	Park H Y,Kang K W,Kim D H,et al.In vitro propagation of Cymbidium goeringii Reichenbach fil.through direct adventitious shoot regeneration[J].Physiology and Molecular Biology of Plants,2018,24(2):307-313.
[30]	Nayak N R,Rath S P,Patnaik S.In vitro propagation of three epiphytic orchids,Cymbidium aloifolium (L.) Sw.,Dendrobium aphyllum (Roxb.)fisch.and Ddendrobium moschatum (Buch-Ham) Sw.through thidiazuron-induced high frequency shoot proliferation[J].Scientia Horticulturae,1997,71(3-4):243-250.
[31]	Chen Y Q,Liu X,Liu Y Q.In vitro plant regeneration from the immature seeds of Cymbidium faberi[J].Plant Cell,Tissue and Organ Culture,1991,24:65-71.
[32]	Shimasaki K,Uemoto S.Micropropagation of a terrestrial Cymbidium species using rhizomes[J].Plant Cell,Tissue and Organ Culture,2005,81:247-251.
[33]	Jainol J E,Gansau J A.Embryogenic callus induction from leaf tip explants and protocorm-like body formation and shoot proliferation of Dimorphorchis lowii:borneon endemic orchid[J].AGRIVITA,Journal of Agricultural Science,2016,39(1):1-10.
[34]	Singh D K,Babbar S B.In vitro propagation and chemical profiling of Herminium lanceum (Thunb.ex Sw.) Vuijk,a medicinally important orchid,for therapeutically important phenolic acids[J].Plant Biotechnology,2016,33(3):153-160.
[35]	谢娟,李龙,邢玥,等.墨兰‘吴字翠’×墨兰‘红花’杂交后代根状茎增殖及芽分化研究[J].热带作物学报,2020,41(5):985-993.
[36]	李国平,杨鹭生.若干因素对寒兰根状茎诱导、增殖与分化的影响[J].赤峰学院学报(自然科学版),2019,35(8):26-29.
[37]	Zeng S J,Wu K L,Teixeira Da Silva J A,et al.Asymbiotic seed germination,seedling development and reintroduction of Paphiopedilum wardii Sumerh.,an endangered terrestrial orchid[J].Scientia Horticulturae,2012,138:198-209.
[38]	孙玉芬,宁惠娟,张韶伊,等.春兰与大花蕙兰杂交后代根状茎增殖与分化条件[J].浙江农林大学学报,2014,31(1):156-161.
[39]	Wang G,Xu Z,Chia T F.In vitro flowering of Dendrobium candidum[J].Science in China,1997,40(1):35-42.