Abstract: In this study, the leaves of Nageia fleuryi used as materials, the chloroplast genome of Nageia fleuryi was sequenced and a phylogenetic tree was constructed to analyze the structural characteristics. The results showed that the chloroplast genome structure of Nageia fleuryi（GenBank No. OL435123） lacked the reverse repeat（IR） region. The chloroplast genome was 133 870 bp in length, the total GC content was 37%, and the total number of innotated genes was 119, including 80 protein-coding genes, 35 tRNA genes and 4 rRNA genes. The chloroplast genome annotation showed that most of the genes were related to photosynthesis, gene transcription and translation, including 43 photosynthesis related genes, 64 self-replicating genes, 8 other coding protein genes and 4 unknown functional genes. The codon preference analysis showed that a total of 44 623 codons were detected, and leucine（Leu） was the largest codon in the chloroplast base group of Nageia fleuryi, accounting for 10.54%, and the codon prefered to use A/U bases. There were 49 SSRs in the chloroplast genome of Nageia fleuryi, contained 26 single nucleotide, 22 dinucleotide and 1 trinucleotide SSRs. The phylogenetic analysis indicated that the Nageia fleuryi（OL435123.1） and the Nageia nagi（AB830885.1 and LC572146.1） were clustered in the same branch and had a relatively close genetic relationship.