Abstract: In this study, 150 F₂, F_2∶3 offsprings crossed by using tropical maize inbred lines of T32 and QR273 as parents, were selected as materials, and genotypes of F₂ individuals were done by using simplified genome sequencing（GBS） method. While five ear related traits of the ear length（cm）, ear diameter（cm）, ear row number（row）, grain number per row（grain） and ear barren tip（cm） of F_2∶3 families were evaluated under two environments of Zhangye, Gansu and Guiyang, Guizhou. QTL mapping was performed by using the complete interval mapping method. Results showed that, a total of 46 QTL associated with ear-related traits were identified under two environments, distributed on 10 Chl omosomes and explained 1.89%-17.18% of the phenotypic variation, respectively. Six QTL explaining more than 10% of the phenotypic variation of ear traits were identified in this paper. And six candidate genes controlling ear related traits were identified including Zm00001d041072, Zm00001d053048, Zm00001d011355, Zm00001d041073, Zm00001d030086 and Zm00001d030088, which encoded proteins involved in plant growth and development, hormone synthesis, nutrient transport and other biological functions, of which these candidate genes could be used as candidate targets for gene cloning and molecular assisted breeding in the future.