Abstract: 102 local maize germplasm from He’nan Province were artificially inoculated in the field to identify the phenotypic resistance to stalk rot disease. The experimental results showed that 16 germplasm materials exhibited first-grade resistance to stalk rot, 17 germplasm materials exhibited third-grade resistance to stalk rot, 30 germplasm materials exhibited fifth-grade moderate resistance to stalk rot, and 39 germplasm materials exhibited susceptibility and high susceptibility to stalk rot. Among them, materials with resistance or higher account for 62% of the total materials, with medium resistance being the main resistance. The isolation and identification of pathogenic bacteria showed that Fusarium graminearum had the strongest pathogenicity, followed by Fusarium rotiferum, which had the weaker pathogenicity on maize plant growth, and Fusarium mangium weakest. The 33 local germplasm with strong disease resistance identified in the resistance identification experiment of local germplasm had enriched and expanded the genetic basis of resistance genes to stalk rot disease. They can be used as important basic materials for breeding new germplasm with resistance and disease resistance, and had practical value for stalk rot disease selection and disease resistance breeding, providing reference for effective rotation of resistance genes in maize production.