Study on Callus Induction and Adventitious Bud Differentiation from Anther of Eggplant

The anthers of 4 eggplant varieties were used as explants to study the induction of anther callus and adventitious bud differentiation in eggplant. The results showed that Xi’an Lvjia and Yuyi 2 mainly differentiated into yellow-white compact callus, which differentiated more adventitious buds, while Cuilv 2 and Heixianzi differentiated into yellow-white loose callus. Further, both loose and compact callus could differentiate green shoots, but the green shoots differentiated from loose callus had a high deformity rate, which was easy to grow slowly and vitrify. The Yuyi 2 and Xi’an Lvjia were suitable for differentiation on F₁ medium, while Cuilv 2 and Heixianzi were more suitable for differentiation on F₂ medium. Additionally, Cuilu 2, Xi’an Lvjia and Yuyi 2 had the highest differentiation rates at the 5 th transdifferentiation, and Heixianzi had the highest differentiation rates at the 4 th transdifferentiation, which were 20%, 107%, 78% and 20%, respectively.