Free Tuber Induction and Its Genetic Stability Identification of Pinellia ternata by SSR

To explore the subculture conditions of loose Pinellia ternate callus, establish suspension culture system of free P. ternate tuber, and verify whether somaclonal variation occurred during tissue culture, the P. ternate petioles were used as explants to study the effects of different concentrations of sucrose and hormone ratio on the proliferation of loose callus, and single factor test and orthogonal test were designed to screen the suitable conditions for inducing the free P. ternate tuber. Meanwhile, the genetic stability of free tuber was also detected by SSR markers. The results showed that MS+2.0 mg·L^-1 2, 4-D+0.5 mg·L^-16-BA+30 g·L^-1 sucrose+0.6% agar was the optimal medium for the formation of loose P. ternate callus, which could obtain a large number of loose P. ternata callus by subculture with this medium. In addition, the medium suitable for the differentiation of loose callus into free tubers was MS+0.5 mg·L^-1 6-BA+0.5 mg·L^-1 IBA+20 g·L^-1 sucrose, which could induce a large number of free P. ternate tubers for artificial seed production or direct sowing. Finally, it was found that the free P. ternate tuber induced by tissue culture did not change at DNA level, but still maintained the genetic stability of its parents based on SSR markers.