Optimization of Tissue Culture and Rapid Propagation System in Leptodermis oblonga Bunge

For the development of wild Leptodermis oblonga Bunge application in garden greening, with wild Leptodermis oblonga Bunge axillary bud stem section as the test materials, the tissue culture method was adopted, the different types of disinfectant and sterilization time foreign implant bud and the influence of different types of plant growth regulator and concentration on the influence of lateral bud multiplication and rooting culture, in order to establish a wild Leptodermis oblonga Bunge seed rapid propagation system. The results showed that the stem segments of wild Leptodermis oblonga Bunge axillary buds were suitable for disinfection with 75% alcohol for 20 s and 5% NaClO for 12 min. The optimal proliferation medium was MS+ZT 1.0 mg/L, and the optimal rooting medium was 1/2 MS+IBA 0.1 mg/L+NAA 0.05 mg/L. The survival rate of rooting seedlings in peat∶vermiculite∶perlite equalling 3∶1∶1 substrate was more than 90%.