Study on Vitro Micrografting Technology of Tissue Culture Plantlets in Camellia sinensis

Firstly, the study established a micrografting techniques system for the tissue cultured buds of the tea in order to solve the problem of tissue culture roots and traditional marriage problems easy to be affected by the season. Rootless tissue culture shoots were used as scions and cutting-rooted seedlings were used as rootstocks to study the effects of training time of tissue culture seedlings, number of scion leaves, scion top, culture temperature, degree of scion lignification, scion length, concentration of growth regulators, stem size of tissue culture seedlings and rootstocks on the survival rate of micrografting.The results showed that the best seedling training time of tissue culture for 4 days, and the scion with 3 leaves was easy to survive grafting. The scion with the top left germinated faster after grafting. The culture temperature of 25 ℃ was favorable for the survival of grafting. The grafting of lignified scion could avoid dying after grafting. The scion length of 2.1-3.0 cm, and the grafting survival rate was the highest after soaking the scion in 1 mg/L NAA for 5 min. Rootstocks and scions with similar diameter of stem were selected for micrografting with high survival rate, reaching 68.33%.