YANG Ling, SHI Xiao-hua, DU Ling-juan. Effects of Temperature and Gibberellin on Seed Germination of Helleborus foetidusJ. Seed, 2022, 41(10): 114-119,149. DOI: 10.16590/j.cnki.1001-4705.2022.10.114
Citation: YANG Ling, SHI Xiao-hua, DU Ling-juan. Effects of Temperature and Gibberellin on Seed Germination of Helleborus foetidusJ. Seed, 2022, 41(10): 114-119,149. DOI: 10.16590/j.cnki.1001-4705.2022.10.114

Effects of Temperature and Gibberellin on Seed Germination of Helleborus foetidus

  • In order to improve the germination rate of Helleborus thibetanus, taking the seeds of Helleborus foetidus as the experimental object, the morphological characteristics of the seeds were observed through stereomicroscope, and the morphological indexes such as water content and water absorption were measured at the same time. By setting different gradients of temperature and gibberellin concentration, the effects of gibberellin and temperature treatment on the seed germination of Helleborus foetidus were studied by petri dish filter paper method. The results showed that the average length of the Helleborus foetidus was 4.435±0.254 mm, the width was 2.407±0.214 mm, and the 1000-seed weight was 13.990±0.070 g; The highest germination rate of seeds was 60% after treatments consisted of 10 weeks of warm at 25 °C followed by 2 weeks of cold at 4 ℃. Gibberellin treatment with mass concentration of 400 mg/L could significantly improve the germination rate of Helleborus foetidus seeds, reaching 73.3%.
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