Establishment of Tissue Culture and Rapid Propagation System of Chinese Medicine Anemarrhena asphodeloides

To establish an efficient tissue culture and rapid propagation system of A. asphodeloides, the leaves, rhizomes and young scapes of A. asphodeloides were inoculated on 9 kinds of combined medium to screen the best explant materials. With MS medium as the basic medium, the medium combinations of different stages of in vitro tissue culture and rapid propagation of A. asphodeloides were screened out through the ratio test of different kinds of hormones and concentrations. The content of mangiferin in tissue culture seedlings of A. asphodeloides which were successfully transplanted under different kinds of hormone and concentration combination medium was determined. Results showed that the young scape was the best explant for callus induction. The medium combination for callus induction was MS+1.0 mg/L KT+0.5 mg/L NAA, and the callus induction rate was 79.40%. The medium combination for inducing callus to produce adventitious buds was MS+0.5 mg/L 6-BA+ 0.5 mg/L NAA, and the bud rate was 100%. The medium combination for rooting culture of adventitious buds was MS+0.5 mg/L NAA, and the rooting rate was 100%. Under this medium combination, the content of mangiferin in the tissue culture seedlings of A. asphodeloides was the highest.