Analysis on ARF-Aux/IAA Interaction Involved in Pericarp Development of Camellia oleifera

Auxin plays an important regulatory role in plant growth and development. ARF and Aux/IAA are key regulatory factors in auxin mediated signal transduction pathways. Peel thickness directly affects the economic yield of Camellia oleifera, and different germplasms of Camellia oleifera peel thickness has obvious differentiation. In order to further study the regulatory mechanism of Camellia oleifera peel thickness, transcriptomic data of Camellia oleifera peel were analyzed by transcriptomic and bioinformatics systems to screen the CoARF and CoIAA genes that could interact with each other during the development of Camellia oleifera peel. The results showed that a total of 19 CoARF and 17 CoIAA genes were identified in the transcriptome data of Camellia oleifera peel, and 10 pairs of CoARF-CoIAA（8 CoARF genes and 6 CoIAA genes） showed significant correlation in temporal and spatial expression. After phylogenetic tree was constructed with ARFs and IAAs in Arabidopsis thaliana, it was found that CoARF14, CoARF19 and CoARF11 were closely related to AtARFs, while CoARF6 and CoARF10 were closely related to AtARFs which played a role in transcriptional activation. After further homologous mapping verification with Arabidopsis thaliana as reference, it was finally determined that the three pairs of CoARF10-CoIAA5, CoARF6-CoIAA2 and CoARF14-CoIAA12 could be involved in regulating the development of Camellia oleifera skin through interaction to transmit auxin signals.