Construction of Fingerprint and Purity Identification of Main Cultivars of Spring Brassica napus in Qinghai Province

A total of 16 SSR markers with polymorphism, clear bands and good repeatability were selected from 190 pairs of Brassica napus SSR primers. 16 pairs of core primers were used to construct fingerprints of 15 parent materials of spring Brassica napus varieties. The results showed that a total of 44 loci were amplified by 16 markers, with an average amplification of 2.5 loci per primer pair. The 15 materials were classified into three classes by UPGMA at a genetic distance of 0.65. Two pairs of SSR markers（A04-10, A06-10） were used, and the markers were closely linked to the Polymaa cytoplasmic male sterility restoration gene（Rfp） YSSR3 was used to identify the purity of the hybrids of Qingza No.4, Qingza No.7, Qingza-limited No.1, Qingza No.5, Qingza No.9, Qingza No.15, Qingza No.12, Qingza No.16 and Qingza No.18, which showed that SSR markers were more advantageous for the purity identification of hybrids.