The Effect of Different Culture Media on the Germination of the Sand Fixing Pioneer Plant Stipagrostis pennata Seeds
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Graphical Abstract
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Abstract
In order to explore the optimum medium conditions for seed germination of Stipagrostis pennata, a pioneer plant of sand fixation, MS, N6, 1/2 MS, AGAR and White media were used for seed germination experiments. After high efficiency(AGAR medium) and low efficiency(White) medium were selected, the seeds were tested for soluble sugar, soluble protein, α-amylase, germinal, ababolic acid, ethethylene, jasmonic acid and growth hormone. The results showed that the seed germination rate was the highest in AGAR medium. Before germination, soluble protein content and α-amylase activity continued to increase, soluble sugar content remained stable, and the contents of related plant hormones gibberellin, ababolic acid, ethethylene, jasmonic acid and auxin continued to increase. Before germination, α-amylase activity continued to increase, soluble protein content decreased significantly, gibberellin concentration was significantly lower than agarose medium, and abscisic acid content was higher than agarose medium. The results showed that AGAR medium was the most efficient medium for seed germination of 5 species of Stipagrostis pennata.
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